| 1. | Results high titers of antipeptide antibody were found in the sera from immunized rabbit s throughout the study period in contrast to those from control rabbits
结果与对照组相比，免疫组动物在整个免疫期间血清抗肽抗体效价均高。 |
| 2. | The purposes of this study is to find good methods for getting a quantity high titer serum and immunoglobulin , decrease the produce cost and control the infectious diseases
特种经济动物因为其本身的经济价值很高，一旦发生传染病损失惨重，因此有必要对患传染病的动物进行治疗和紧急预防，将损失降到最小。 |
| 3. | One group was immunized with cdv vaccine , and the other group was cav . serum samples and eggs were collected weekly for detected antibody titers . high titer egg yolk was collected for following igy extraction
分别用cdv 、 cav的疫苗免疫产蛋鸡，每周检测鸡血清和卵黄中的抗体水平，收集抗体水平高的鸡蛋用于提取纯化卵黄抗体igy 。 |
| 4. | The target proteins with molecular weights of 35kd and 44kd were detected by sds - page . the 35kd target protein recovered from sds - page was used to immunize rabbit and the annsemm with high titer was obtained
Annfaf基因在大肠杆菌中以包含体的形式得到了高效表达， sds - page检测表明，分别获得44kd和35kd大小的蛋白带，与预期结果相同。 |
| 5. | Mixed connective tissue disease ( mctd ) was defined as a connective tissue disorder characterized by the presence of high titers of a distinct autoantibody in combination with clinical features commonly seen in systemic lupus erythematosus ( sle ) , scleroderma , and polymyositis ( referred to as overlap syndrome )
摘要混合性结缔组织疾病是一种结缔组织异常的疾病，合并有高价且明确的自体抗体，临床上常有类似全身性红斑性狼疮，硬皮症及多发性肌炎的表现。 |
| 6. | 1 . cloning of the gene encoding the specific ige antibody related antigen of schistosoma japonicum the serum samples from the inhabitants living in the epidemic regions of schistosomaisis japonica were collected and detected by abc - elisa method for their swap specific ige antibody . the sera with high titer of the specific ige levels were chosen and pooled
89 ，其亲水性、抗原性和可及性分布曲线基本一致，预测抗原表位分另ij在第25 35位、 75 82位、 1h 1们位、 117 1n位和149 159位氨基酸残基。 |
| 7. | The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。 |
| 8. | Once the recombinant virus has been i - dentified , we amplified the p - 1 stock to attain the large scale , high - titer viral stock in order to initiate expression studies . the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared . the time course for expression of recombinant protein was detected by sds - page and western blot , which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表达重组蛋白angiostatin ：用制备好的带有矗s标记的融合性angiostatin基因的高滴度重组杆状病毒贮存液感染草地贪夜蛾sfg细胞，用sds page电泳和hsternblot对感染不同时间后分泌的重组蛋白做时间表达分析，依此确定最适的感染复数（ moi ）和感染时间，以达到重组蛋白表达水平最适化，而后大规模进行重组蛋白的表达， sds page用来分析重组蛋白， westernblot用来在蛋白表达水平低的情况下检测表达的特异重组蛋白。 |
| 9. | Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using . triplix kit . a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction . the ss - dna was reversely transcripted from total rna . double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i , the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro . a high titer and high recombinant ratio of cdna library was constructed
构建恶性疟原虫海南株红内期cdna表达文库提取红内期恶性疟原虫海南株总rna ，直接以总rna为模板使用cdna文库构建试剂盒，首先反转录合成ss一dna ，再扩增合成ds一dna ( cdna ) ，对扩增产物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶试剂盒纯化、回收20obp以上的片断，经与载体连接再用蛋白包装物包装后形成未扩增文库，最后扩增完成恶性疟原虫海南株红内期cdna表达文库的构建。 |
| 10. | Anti - melatonin monoclonal antibodies of higher titer , affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections . we obtained 6 strains of hybridoma , all of them secreting specific antibodies to mt , we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique . an inhibition curve for mt was obtained in the range of 50pg to30ng , and 1 . 4ng of mt inhibited the value of the assay by half . we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues , the moabs recognized mt but
通过将mt用甲醛作连结剂连结到牛血清白蛋白上sa采用皮下多点注射兔疫小鼠得到了高效价，高亲和力，较好特异性的抗mt单克隆抗体，最后获得了5株单克隆细胞株，都能分泌针对mt的特异性抗体，建立了选择性基团免疫分析法，用制备的抗体测定了人血清中mt的含量，作了mt的抑制标准曲线，其抑制范围从50pg ? 30ng ，半抑制量为1 |
